In the radioimmunoassay, one can radiolabel a particular antigen or antibody using either I or C tagged to the antigen or antibody. Once the serum or purified antibody or antigen to be tested is placed in the well, a second radiolabel antihuman IgG antibody is placed in the well. After appropriate binding and further washes, the degree of activity of the antibody to a given antigen can be determined in a γ counter.
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