The method is relatively straightforward. One takes the tissues or cells to be studied and prepares them as follows;
Tissue-->mRNA-->cDNA-->Biotinylated cRNA
The bioinylated material is layered on glasss slides on which DNA fragments of the human genome have been placed (12.000 probe sets/clip). The chips are then washed, stained with streptavidin-phycoerythrin, and scanned with a probe array scanner.
No comments:
Post a Comment