The lymphocytes are identified with anti-CD3 labels. Of this population, the number of B cells in the population is identified by CD19-PE labeled antibodies. Finally, the number of B cells expressing a novel antigen is identified, in which the percentage of CD19+ exhibiting D8/17+ B cells is calculated. All of these values are obtained rapidly due to the speed with which cells are counted with different labels, and the results are accurate because of the large number of cells that are counted (in the thousands). It should be emphasized that values for lymphocyte subsets will change with increasing age from children to adults, especially during the first year of life.
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