The main principle behind this test is related to the formation of immune complexes between the antibody and a given antigen. If the concentration of antigen-antibody complex is low, then the immune complexes remain in suspension as fine particles, which can disperse a beam of light. As the complexes increase with the concentration of antibody, the complexes will precipitate, and light scattering will decrease. This degree of dispersion can be measuired on a nephelometer.
No comments:
Post a Comment