HLA antibody can also be detected in an indirect ELISA; however, this is less sensitive than bead platforms. From a technical perspective. single antigen beads assays allow identification of HLA antibodies for all common and many rare antigens and alleles at up to 11 HLA loci. Single antigen beads assays are rapid (3-6 hours), with up to 100 unique antigen beads able to be tested in a single reaction chamber, and high throughput, with additional multiplexing ability to test many patients simultaneously.
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